A rapid and quantitative method for the evaluation of V gene usage, specificities and the clonal size of B cell repertoires.
J Immunol Methods
; 376(1-2): 143-9, 2012 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-22226792
ABSTRACT
The quantitative simultaneous description of both variable region gene usage and antigen specificity of immunoglobulin repertoires is a major goal in immunology. Current quantitative assays are labor intensive and depend on extensive gene expression cloning prior to screening for antigen specificity. Here we describe an alternative method based on high efficiency single B cell cultures coupled with RT-PCR that can be used for rapid characterization of immunoglobulin gene segment usage, clonal size and antigen specificity. This simplified approach should facilitate the study of antibody repertoires expressed by defined B cell subpopulations, the analysis of immune responses to self and nonself-antigens, the development and screening of synthetic antibodies and the accelerated study and screening of neutralizing antibodies to pathogenic threats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Região Variável de Imunoglobulina
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Linfócitos B
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Células Clonais
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Clonagem Molecular
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article