A homogeneous assay for protein analysis in droplets by fluorescence polarization.
Electrophoresis
; 33(3): 436-9, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-22228311
ABSTRACT
We present a novel homogeneous ("mix-incubate-read") droplet microfluidic assay for specific protein detection in picoliter volumes by fluorescence polarization (FP), for the first time demonstrating the use of FP in a droplet microfluidic assay. Using an FP-based assay we detect streptavidin concentrations as low as 500 nM and demonstrate that an FP assay allows us to distinguish droplets containing 5 µM rabbit IgG from droplets without IgG with an accuracy of 95%, levels relevant for hybridoma screening. This adds to the repertoire of droplet assay techniques a direct protein detection method which can be performed entirely inside droplets without the need for labeling of the analyte molecules.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Técnicas Analíticas Microfluídicas
/
Polarização de Fluorescência
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article