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Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis.
Procházková, Katerina; Cermáková, Katerina; Pachl, Petr; Sieglová, Irena; Fábry, Milan; Otwinowski, Zbyszek; Rezácová, Pavlína.
Afiliação
  • Procházková K; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nam. 2, Prague 6, Czech Republic.
Acta Crystallogr D Biol Crystallogr ; 68(Pt 2): 176-85, 2012 Feb.
Article em En | MEDLINE | ID: mdl-22281747
In Bacillus subtilis, the arabinose repressor AraR negatively controls the expression of genes in the metabolic pathway of arabinose-containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: an N-terminal DNA-binding domain belonging to the GntR family and a C-terminal effector-binding domain that shows similarity to members of the GalR/LacI family. The crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose has been determined at 2.2 Šresolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry; the K(d) value was 8.4 ± 0.4 µM. The effect of L-arabinose on the protein oligomeric state was investigated in solution and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Bacillus subtilis / Proteínas de Bactérias Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Bacillus subtilis / Proteínas de Bactérias Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2012 Tipo de documento: Article