Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA).
Bioinformatics
; 28(6): 884-5, 2012 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-22285829
ABSTRACT
Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R/Bioconductor software package DTA. It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Perfilação da Expressão Gênica
/
Estudo de Associação Genômica Ampla
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article