Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.
J Struct Funct Genomics
; 13(2): 71-9, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22286688
ABSTRACT
Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins' functions were obtained from their interactions with each of a panel of ligands.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triazinas
/
Cromatografia de Afinidade
/
Cristalografia por Raios X
/
Nucleotídeos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article