Early tissue transglutaminase-mediated response underlies K562(S)-cell gliadin-dependent agglutination.
Pediatr Res
; 71(5): 532-8, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22314661
ABSTRACT
INTRODUCTION:
[corrected] K562(S) agglutination has been used as a rapid and economic tool for the in vitro screening of the toxicity of cereal fractions and prolamins in celiac disease (CD). A strict correlation has been reported between the toxicity of cereals and cereal fractions for celiac patients and their ability to agglutinate K562(S) cells. Whether this specificity of K562(S)-cell agglutination is caused by the activation of the same pathogenic events triggered by toxic cereal fractions in CD intestine or simply represents a bystander event of gluten toxicity is, however, unknown.METHODS:
K562(S) cells were incubated in vitro with the peptic-tryptic digest of wheat gliadin.RESULTS:
The agglutination of K562(S) cells by wheat gliadin peptides is orchestrated by a cascade of very early events occurring at the K562(S)-cell surface similar to those occurring at the intestinal epithelial surface. They involve a rapid increase in intracellular calcium levels that activate tissue transglutaminase (TG2), leading to a rapid actin reorganization that is pivotal in driving cell agglutination. These specific effects of toxic cereals are phenocopied by the gliadin-derived peptide p31-43, which orchestrates the activation of innate response to gliadin in CD.DISCUSSION:
Our study provides the rationale for the extensive use of K562(S)-cell agglutination as a valuable tool for screening cereal toxicity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adesão Celular
/
Transglutaminases
/
Gliadina
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article