A sterol-binding protein integrates endosomal lipid metabolism with TOR signaling and nitrogen sensing.
Cell
; 148(4): 702-15, 2012 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-22341443
ABSTRACT
Kes1, and other oxysterol-binding protein superfamily members, are involved in membrane and lipid trafficking through trans-Golgi network (TGN) and endosomal systems. We demonstrate that Kes1 represents a sterol-regulated antagonist of TGN/endosomal phosphatidylinositol-4-phosphate signaling. This regulation modulates TOR activation by amino acids and dampens gene expression driven by Gcn4, the primary transcriptional activator of the general amino acid control regulon. Kes1-mediated repression of Gcn4 transcription factor activity is characterized by nonproductive Gcn4 binding to its target sequences, involves TGN/endosome-derived sphingolipid signaling, and requires activity of the cyclin-dependent kinase 8 (CDK8) module of the enigmatic "large Mediator" complex. These data describe a pathway by which Kes1 integrates lipid metabolism with TORC1 signaling and nitrogen sensing.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endossomos
/
Saccharomyces cerevisiae
/
Transdução de Sinais
/
Metabolismo dos Lipídeos
/
Nitrogênio
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article