Development and evaluation of a PCR assay for tracking the emergence and dissemination of Haitian variant ctxB in Vibrio cholerae O1 strains isolated from Kolkata, India.
J Clin Microbiol
; 50(5): 1733-6, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22357499
ABSTRACT
A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of ctxB alleles. Our retrospective study using this newly developed PCR showed that Haitian ctxB first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant strains isolated recently that was distinct from the PFGE pattern of the strains carrying classical ctxB that closely matched the 2006 to 2007 variant strains.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cólera
/
Toxina da Cólera
/
Reação em Cadeia da Polimerase
/
Vibrio cholerae O1
Tipo de estudo:
Evaluation_studies
/
Observational_studies
Limite:
Humans
País/Região como assunto:
Asia
/
Haiti
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article