CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.
J Infect Dis
; 205(7): 1095-9, 2012 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-22357696
ABSTRACT
We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sarcoma de Kaposi
/
DNA Viral
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DNA-Citosina Metilases
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Herpesvirus Humano 8
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Linfoma de Efusão Primária
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article