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Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool.
Christy, Nathaniel C V; Hencke, Janice D; Escueta-De Cadiz, Aleyla; Nazib, Forida; von Thien, Heidrun; Yagita, Kenji; Ligaba, Segni; Haque, Rashidul; Nozaki, Tomoyoshi; Tannich, Egbert; Herbein, Joel F; Petri, William A.
Afiliação
  • Christy NC; Division of Infectious Diseases and International Health, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
J Clin Microbiol ; 50(5): 1762-3, 2012 May.
Article em En | MEDLINE | ID: mdl-22378909
A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parasitologia / Técnicas de Laboratório Clínico / Cryptosporidium / Entamoeba histolytica / Fezes / Giardia / Antígenos de Protozoários Tipo de estudo: Clinical_trials / Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parasitologia / Técnicas de Laboratório Clínico / Cryptosporidium / Entamoeba histolytica / Fezes / Giardia / Antígenos de Protozoários Tipo de estudo: Clinical_trials / Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Ano de publicação: 2012 Tipo de documento: Article