Multiplexed suspension array platform for high-throughput protein assays.
Biomed Microdevices
; 14(4): 651-7, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22391879
ABSTRACT
A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experimental system consists of a microfluidic chamber for arraying the particles in a manner suitable for high throughput imaging using a simple fluorescent microscope, together with custom software for automated code readout and analysis of assay response. The platform is demonstrated with a multiplexed antibody assay targeting 3 different human inflammatory cytokines. The suitability of the platform for other bio-analytical applications is discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Serial de Proteínas
/
Ensaios de Triagem em Larga Escala
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article