Development of an amine dehydrogenase for synthesis of chiral amines.
Angew Chem Int Ed Engl
; 51(16): 3969-72, 2012 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-22396126
ABSTRACT
A leucine dehydrogenase has been successfully altered through several rounds of protein engineering to an enantioselective amine dehydrogenase. Instead of the wild-type α-keto acid, the new amine dehydrogenase now accepts the analogous ketone, methyl isobutyl ketone (MIBK), which corresponds to exchange of the carboxy group by a methyl group to produce chiral (R)-1,3-dimethylbutylamine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases atuantes sobre Doadores de Grupo CH-NH
/
Aminas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article