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A new mechanism to ensure integration during LINE retrotransposition: a suggestion from analyses of the 5' extra nucleotides.
Kajikawa, Masaki; Yamaguchi, Katsumi; Okada, Norihiro.
Afiliação
  • Kajikawa M; Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-15 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan. mkaji@bio.titech.ac.jp
Gene ; 505(2): 345-51, 2012 Sep 01.
Article em En | MEDLINE | ID: mdl-22405943
ABSTRACT
Long interspersed elements (LINEs) are transposable elements that exist in the chromosomal DNA of most eukaryotes; as such, they have a large impact on the genome evolution of their hosts. LINEs mobilize by a mechanism called retrotransposition in which the LINE RNA is reverse-transcribed into DNA and then integrated into the host chromosome. The integration of the 3' end of the LINE element simultaneously occurs with the initiation of reverse transcription; this process is called target-primed reverse transcription and is one of the important characteristics of LINEs. However, the molecular mechanism of the integration of the 5' end is not well understood. Here, we show that, in cultured cells, the integrants of the zebrafish ZfL2-2 LINE produce extra nucleotides at their 5' ends, and the extra nucleotides originate from their flanking sequences. We also found that, in cultured cells, some integrants of the human L1 LINE and, in their native hosts, some endogenous elements of two other LINEs also contain 5' extra nucleotides of similar origin, suggesting that the mechanism for generation of the 5' extra nucleotides is universal among various LINEs. From these data, we propose a general mechanism for 5' integration in LINE retrotransposition.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Elementos Nucleotídeos Longos e Dispersos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Elementos Nucleotídeos Longos e Dispersos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article