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Comparative analysis of tumor markers and evaluation of their predictive value in patients with colorectal cancer.
Liu, Zhi-Ping; Li, Li-Min; Liu, Xiu-Li; Zhang, Da-Xin.
Afiliação
  • Liu ZP; Department of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin, PR China.
Onkologie ; 35(3): 108-13, 2012.
Article em En | MEDLINE | ID: mdl-22414974
ABSTRACT

BACKGROUND:

We evaluated the feasibility of CEA/CK20 mRNA and CEA/CA19-9 proteins as tumor markers for colorectal cancer by detecting tumor-specific mRNAs in circulating tumor cells and secreted tumor-specific proteins in the peripheral blood of colorectal cancer patients. PATIENTS AND

METHODS:

Peripheral blood was obtained from 23 healthy volunteers and 46 colorectal cancer patients on the day of initiation of adjuvant chemotherapy after surgery (stages I-III, n = 27) or on the first day of chemotherapy after diagnosis (stage IV, n = 19). Levels of CEA/CK20 mRNA in peripheral blood mononuclear cells (PBMCs) were determined with quantitative real-time reverse transcription polymerase chain reaction, and serum CEA/CA19-9 protein levels were determined by radioimmunoassay.

RESULTS:

The detection sensitivity of CK20 mRNA was approximately 1 tumor cell in 1 × 10(7) PBMCs, and that of CEA mRNA was approximately 1 tumor cell in 1 × 10(6) PBMCs. Patients with stage IV colorectal cancer had higher levels of CEA mRNA, CK20 mRNA, and serum CEA than patients at stages I-III. Peripheral blood CEA mRNA levels were predictive of overall survival, while serum protein levels of CEA and CA19-9 had no predictive value.

CONCLUSION:

Peripheral blood CEA mRNA is a useful marker of overall survival in colorectal cancer patients, that is sensitive and specific.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / RNA Neoplásico / Neoplasias Colorretais / Antígeno Carcinoembrionário / Biomarcadores Tumorais / Antígeno CA-19-9 / Queratina-3 Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / RNA Neoplásico / Neoplasias Colorretais / Antígeno Carcinoembrionário / Biomarcadores Tumorais / Antígeno CA-19-9 / Queratina-3 Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2012 Tipo de documento: Article