Comparative analysis of tumor markers and evaluation of their predictive value in patients with colorectal cancer.
Onkologie
; 35(3): 108-13, 2012.
Article
em En
| MEDLINE
| ID: mdl-22414974
ABSTRACT
BACKGROUND:
We evaluated the feasibility of CEA/CK20 mRNA and CEA/CA19-9 proteins as tumor markers for colorectal cancer by detecting tumor-specific mRNAs in circulating tumor cells and secreted tumor-specific proteins in the peripheral blood of colorectal cancer patients. PATIENTS ANDMETHODS:
Peripheral blood was obtained from 23 healthy volunteers and 46 colorectal cancer patients on the day of initiation of adjuvant chemotherapy after surgery (stages I-III, n = 27) or on the first day of chemotherapy after diagnosis (stage IV, n = 19). Levels of CEA/CK20 mRNA in peripheral blood mononuclear cells (PBMCs) were determined with quantitative real-time reverse transcription polymerase chain reaction, and serum CEA/CA19-9 protein levels were determined by radioimmunoassay.RESULTS:
The detection sensitivity of CK20 mRNA was approximately 1 tumor cell in 1 × 10(7) PBMCs, and that of CEA mRNA was approximately 1 tumor cell in 1 × 10(6) PBMCs. Patients with stage IV colorectal cancer had higher levels of CEA mRNA, CK20 mRNA, and serum CEA than patients at stages I-III. Peripheral blood CEA mRNA levels were predictive of overall survival, while serum protein levels of CEA and CA19-9 had no predictive value.CONCLUSION:
Peripheral blood CEA mRNA is a useful marker of overall survival in colorectal cancer patients, that is sensitive and specific.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
RNA Neoplásico
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Neoplasias Colorretais
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Antígeno Carcinoembrionário
/
Biomarcadores Tumorais
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Antígeno CA-19-9
/
Queratina-3
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article