[Simultaneous determination of 11 phthalate metabolites in urine by liquid chromatography tandem mass spectrometry].

ABSTRACT

OBJECTIVE: To develop a method for determining 11 kinds of phthalate metabolites in urine simultaneously. METHODS: The phthalate metabolites in human urine samples were hydrolyzed by glucuronidase, and then were concentrated by solid-phase extraction and eluted by methanol/acetonitrile (1 1, V/V). After chromatographic separation on phenyl column, the analytes were analyzed qualitatively and quantitatively under MRM mode. RESULTS: The limits of detection (LOD) for the eleven kinds of phthalate metabolites were in the range of 0.13 - 2.27 ng/ml. The mean recoveries at the two spiked levels were 67% - 108%, with the relative standard deviation (RSD) of less than 12%. CONCLUSION: The method can be used to determine the residues of eleven kinds of phthalate metabolites in urine accurately and sensitively.