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The PhoP/PhoQ system and its role in Serratia marcescens pathogenesis.
Barchiesi, Julieta; Castelli, María Eugenia; Di Venanzio, Gisela; Colombo, María Isabel; García Véscovi, Eleonora.
Afiliação
  • Barchiesi J; Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
J Bacteriol ; 194(11): 2949-61, 2012 Jun.
Article em En | MEDLINE | ID: mdl-22467788
Serratia marcescens is able to invade, persist, and multiply inside nonphagocytic cells, residing in nonacidic, nondegradative, autophagosome-like vacuoles. In this work, we have examined the physiological role of the PhoP/PhoQ system and its function in the control of critical virulence phenotypes in S. marcescens. We have demonstrated the involvement of the PhoP/PhoQ system in the adaptation of this bacterium to growth on scarce environmental Mg(2+), at acidic pH, and in the presence of polymyxin B. We have also shown that these environmental conditions constitute signals that activate the PhoP/PhoQ system. We have found that the two S. marcescens mgtE orthologs present a conserved PhoP-binding motif and demonstrated that mgtE1 expression is PhoP dependent, reinforcing the importance of PhoP control in magnesium homeostasis. Finally, we have demonstrated that phoP expression is activated intracellularly and that a phoP mutant strain is defective in survival inside epithelial cells. We have shown that the Serratia PhoP/PhoQ system is involved in prevention of the delivery to degradative/acidic compartments.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Proteínas de Bactérias / Infecções por Serratia Tipo de estudo: Etiology_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Proteínas de Bactérias / Infecções por Serratia Tipo de estudo: Etiology_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article