Sequence in the influenza A virus nucleoprotein required for viral polymerase binding and RNA synthesis.
J Virol
; 86(13): 7292-7, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22532672
ABSTRACT
Many proposed mechanisms for influenza A viral RNA synthesis include an interaction of the nucleoprotein (NP) with the viral polymerase. To identify an NP sequence required for this interaction, we used the cryoelectron microscopic structure of an influenza virus miniribonucleoprotein as a guide for choosing promising surface-exposed sequences. We show that three amino acids (R204, W207, and R208) located in a loop at the top of the head domain of NP are required for functional interaction with the viral polymerase. Quantitative reverse transcription-PCR (RT-PCR) measurements of RNAs synthesized in minigenome assays established that each of these NP amino acids is required for viral RNA synthesis. The mutation of these three amino acids does not affect nuclear localization or RNA-binding and oligomerization activities of NP. In vitro binding experiments with purified virus polymerase and NPs established that these three amino acids are required for NP binding to the viral polymerase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Replicação Viral
/
RNA Polimerase Dependente de RNA
/
RNA Viral
/
Proteínas do Core Viral
/
Proteínas de Ligação a RNA
/
Domínios e Motivos de Interação entre Proteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article