Cell-specific susceptibility to prion strains is a property of the intact cell.
Prion
; 6(4): 371-4, 2012.
Article
em En
| MEDLINE
| ID: mdl-22561192
ABSTRACT
Prions consist of PrP (Sc), a misfolded version of the cellular protein PrP (C). They occur in a variety of strains that share the amino acid sequence of PrP but differ in phenotypic properties, such as cell tropism and pathogenicity; strain-ness is attributed to the conformation of PrP (Sc). To gain insight as to how susceptibility of cells to a given prion strain comes about, we compared amplification of RML prions by PMCA, using cell lysates from related, RML-resistant and RML-susceptible cell lines as substrate. We found that both lysates supported amplification of RML PrP (Sc) equally well, despite a 280-fold difference in the susceptibility of the cells from which they were derived. Thus, susceptibility is an attribute of the intact cell.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Priônicas
/
Proteínas PrPSc
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article