Rational design of true monomeric and bright photoactivatable fluorescent proteins.
Nat Methods
; 9(7): 727-9, 2012 May 13.
Article
em En
| MEDLINE
| ID: mdl-22581370
ABSTRACT
Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations and forms aggregates when labeling membrane proteins, limiting its application as a fusion partner. We solved the crystal structure of tetrameric mEos2 and rationally designed improved versions, mEos3.1 and mEos3.2, that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia de Proteínas
/
Proteínas de Fluorescência Verde
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Proteínas Luminescentes
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Microscopia de Fluorescência
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article