Proficiency of PCR in hospital settings for nonculture diagnosis of invasive meningococcal infections.
Clin Lab
; 58(3-4): 343-6, 2012.
Article
em En
| MEDLINE
| ID: mdl-22582511
ABSTRACT
BACKGROUND:
Meningococcal meningitis requires rapid diagnosis and immediate management which is enhanced by the use of PCR for the ascertainment of these infections. However, its use is still restricted to reference laboratories.METHODS:
We conducted an inter-laboratory study to assess the implementation and the performance of PCR in ten French hospital settings in 2010.RESULTS:
Our data are in favour of this implementation. Although good performance was obtained in identifying Neisseria meningitidis positive samples, the main issue was reported in identifying other species (Streptococcus pneumoniae and Haemophilus influenzae) which are also involved in bacterial meningitis cases.CONCLUSIONS:
Several recommendations are required and, mainly, PCR should target the major etiological agents (N. meningitidis, S. pneumonia, and H. influenzae) of acute bacterial meningitis. Moreover, PCR should predict the most frequent serogroups of Neisseria meningitidis according to local epidemiology.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Meningite Meningocócica
/
Neisseria meningitidis
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Guideline
/
Prognostic_studies
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article