Inhibition of RhoA/ROCK signaling pathway promotes the apoptosis of gastric cancer cells.
Hepatogastroenterology
; 59(120): 2523-6, 2012.
Article
em En
| MEDLINE
| ID: mdl-22584506
ABSTRACT
BACKGROUND/AIMS:
Ras homologue A (RhoA) plays a crucial role in the proliferation, apoptosis,adhesion and migration of gastric cancer cells. Rho associated kinase (ROCK) is an effector protein of RhoA.METHODOLOGY:
In the present study, RhoA activity was inhibited by siRNA targeting RhoA andY-27632, an inhibitor of ROCK, and the role of RhoA/ROCK signaling pathway in the apoptosis of gastric cancer cells was investigated.RESULTS:
RNAi of RhoA inhibited the survival and promoted the apoptotic of AGS cells. RhoA RNAi caused an obvious decrease of ROCK1 expression but an increase of caspase-3/cleaved-caspase-8. Inhibition of ROCK by Y-27632 inhibited the activity of RhoA and promoted the apoptosis.CONCLUSIONS:
We speculate that RhoA/ROCK signaling pathway plays an important role in the regulation of apoptosis of gastric cancer, and to inhibit this pathway may promote the apoptosis of cancer cells. Thus, inhibition of RhoA/ROCK signaling pathway may become a novel target in the treatment of gastric cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piridinas
/
Neoplasias Gástricas
/
Transdução de Sinais
/
Apoptose
/
Proteína rhoA de Ligação ao GTP
/
Interferência de RNA
/
Inibidores de Proteínas Quinases
/
Quinases Associadas a rho
/
Amidas
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article