Electrophoretic separation and detection of metalloproteins by X-ray fluorescence mapping.
Methods Mol Biol
; 869: 533-42, 2012.
Article
em En
| MEDLINE
| ID: mdl-22585518
ABSTRACT
All living systems depend on metalloproteins. Yet, while tools for the separation and identification of apo-proteins are well developed, those enabling identification and quantitation of individual metalloproteins within complex mixtures are still nascent. Here, we describe the electrophoretic separation of a mixture of carbonic anhydrase, ceruloplasmin, urease, and hemoglobin using native 2D gel electrophoresis and X-ray fluorescence mapping-an approach we have developed to be broadly applicable, not require specialized equipment for sample preparation, and likely to be extensible in the future.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria por Raios X
/
Urease
/
Ceruloplasmina
/
Hemoglobinas
/
Anidrases Carbônicas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article