Staining-free gel electrophoresis-based multiplex enzyme assay using DNA and peptide dual-functionalized gold nanoparticles.
Electrophoresis
; 33(8): 1288-91, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22589108
ABSTRACT
We report a simple staining-free gel electrophoresis method to simultaneously probe protease and nuclease. Utilizing gold nanoparticles (Au-NPs) dual-functionalized with DNA and peptide, the presence and concentration of nuclease and protease are determined concurrently from the relative position and intensity of the bands in the staining-free gel electrophoresis. The use of Au-NPs eliminates the need for staining processes and enables naked eye detection, while a mononucleotide-mediated approach facilitates the synthesis of DNA/peptide conjugated Au-NPs and simplifies the operation procedures. Multiplex detection and quantification of DNase I and trypsin are successfully demonstrated.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
DNA
/
Eletroforese
/
Nanopartículas Metálicas
/
Ensaios Enzimáticos
/
Ouro
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article