Neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine.
J Clin Periodontol
; 39(7): 626-34, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22607095
ABSTRACT
AIM:
To determine the effect of cigarette smoke extract, nicotine and cotinine on lucigenin-detectable neutrophil superoxide production. MATERIALS &METHODS:
Neutrophils from periodontally healthy individuals were treated with aqueous smoke extract, nicotine and cotinine, prior to stimulation or at the same time as stimulation with Fusobacterium nucleatum, IgG-opsonized Staphylococcus aureus and Escherichia coli Lipopolysaccharide (LPS). Superoxide generation was determined by lucigenin chemiluminescence.RESULTS:
Smoke extract induced superoxide release from neutrophils (p <0.0001) in a dose-dependent manner. By contrast, superoxide generation by neutrophils in response to pathologically relevant stimuli was inhibited by pre-treatment with smoke extract (p <0.01). This inhibition did not require the continued presence of the extract. A similar reduction in stimulated superoxide production by smoke extract was detected when neutrophils were simultaneously exposed to the extract and stimuli. Nicotine and cotinine (0-10 µg/ml) had no effect on superoxide release from unstimulated or stimulated neutrophils.CONCLUSIONS:
Stable water-soluble components of cigarette smoke directly induce superoxide generation by otherwise unstimulated neutrophils, but reduce superoxide responses of cells to pathologically relevant stimuli. These data suggest potential neutrophil-mediated mechanisms by which smoking may initiate and maintain oxidative stress at periodontally healthy sites and participate in disease progression, by reducing innate immune responses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fumaça
/
Nicotiana
/
Oxidantes
/
Superóxidos
/
Agonistas Nicotínicos
/
Cotinina
/
Misturas Complexas
/
Neutrófilos
/
Nicotina
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article