Blockade of ß-cell K(ATP) channels by the endocannabinoid, 2-arachidonoylglycerol.
Biochem Biophys Res Commun
; 423(1): 13-8, 2012 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-22609205
ABSTRACT
The endocannabinoid system has been demonstrated to be active in the pancreatic ß-cell. However the effects of the endocannabinoids (ECs) on insulin secretion are not well defined and may vary depending on the metabolic state of the ß-cell. Specifically it is not known whether the effects of the ECs occur by activation of the cannabinoid receptors or via their direct interaction with the ion channels of the ß-cell. To begin to delineate the effects of ECs on ß-cell function, we examined how the EC, 2-AG influences ß-cell ion channels in the absence of glucose stimulation. The mouse insulinoma cell line R7T1 was used to survey the effects of 2-AG on the high voltage activated (HVA) calcium, the delayed rectifier (K(v)), and the ATP-sensitive K (K(ATP)) channels by whole cell patch clamp recording. At 2mM glucose, 2-AG inhibited the HVA calcium (the majority of which are L-type channels), K(v), and K(ATP) channels. The channel exhibiting the most sensitivity to 2-AG blockade was the K(ATP) channel, where the IC(50) for 2-AG was 1 µM. Pharmacological agents revealed that the blockade of all these channels was independent of cannabinoid receptors. Our results provide a mechanism for the previous observations that CB1R agonists increase insulin secretion at low glucose concentrations through CB1R independent blockade of the K(ATP) channel.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Araquidônicos
/
Endocanabinoides
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Células Secretoras de Insulina
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Canais KATP
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Moduladores de Receptores de Canabinoides
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Glicerídeos
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Insulina
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article