Halobaculum magnesiiphilum sp. nov., a magnesium-dependent haloarchaeon isolated from commercial salt.
Int J Syst Evol Microbiol
; 63(Pt 3): 861-866, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-22611203
ABSTRACT
Two extremely halophilic archaea, strains MGY-184(T) and MGY-205, were isolated from sea salt produced in Japan and rock salt imported from Bolivia, respectively. Both strains were pleomorphic, non-motile, Gram-negative and required more than 5â% (w/v) NaCl for growth, with optimum at 9-12â%, in the presence of 2â% (w/v) MgCl2â.â6H2O. In the presence of 18â% (w/v) MgCl2â.â6H2O, however, both strains showed growth even at 1.0â% (w/v) NaCl. Both strains possessed two 16S rRNA genes (rrnA and rrnB), and they revealed closest similarity to Halobaculum gomorrense JCM 9908(T), the single species with a validly published name of the genus Halobaculum, with similarity of 97.8â%. The rrnA and rrnB genes of both strains were 100â% similar. The rrnA genes were 97.6â% similar to the rrnB genes in both strains. DNA G+C contents of strains MGY-184(T) and MGY-205 were 67.0 and 67.4 mol%, respectively. Polar lipid analysis revealed that the two strains contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester derived from C20C20 archaeol. The DNA-DNA hybridization value between the two strains was 70â% and both strains showed low levels of DNA-DNA relatedness (48-50â%) with Halobaculum gomorrense JCM 9908(T). Physiological and biochemical characteristics allowed differentiation of strains MGY-184(T) and MGY-205 from Halobaculum gomorrense JCM 9908(T). Therefore, strains MGY-184(T) and MGY-205 represent a novel species of the genus Halobaculum, for which the name Halobaculum magnesiiphilum sp. nov. is proposed; the type strain is MGY-184(T) (â=âJCM 17821(T)â=âKCTC 4100(T)).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Cloreto de Sódio
/
Halobacteriaceae
País/Região como assunto:
America do sul
/
Asia
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Bolivia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article