[Simultaneous determination of six antibiotics in disinfection products by high performance liquid chromatography tandem mass spectrometry].
Wei Sheng Yan Jiu
; 41(2): 282-6, 2012 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-22611942
ABSTRACT
OBJECTIVE:
To develop a method for determination of the Metronidazole, Chlortetracycline hydrochloride, Oxytetracycline dihydrate, Minocycline hydrochloride, Erythromycin and Tetracycline hydrochloride in disinfection products by high performance liquid chromatography tandem mass spectrometry(LC-MS/MS).METHODS:
Samples were extracted by methanol and filtered through 0.45 microm PTFE membrane filter, then analyzed by LC-MS/MS using Waters Symmetry C18 (2.1 mm x 150 mm, 3.5 microm) column in positive ion scan mode. The mobile phase was 5 mmol/L ammonium acetate, methanol and acetonitrile.RESULTS:
The linear range was 0-2000 ng/ml and the correlation coefficients were more than 0.998, the average recoveries ranged from 74.7% to 114% with the relative standard deviations between 1.6%-20.2%. The method was successfully used to detect the content of antibiotics in 115 disinfection products.CONCLUSION:
The method is simple, sensitive, selective and suitable for the analysis of residual content of antibiotics in cream formulations of disinfection products.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pomadas
/
Clortetraciclina
/
Desinfetantes
/
Metronidazol
/
Antibacterianos
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article