Enhanced T cell immunity by B7-H4 downregulation in nonsmall-cell lung cancer cell lines.
J Int Med Res
; 40(2): 497-506, 2012.
Article
em En
| MEDLINE
| ID: mdl-22613410
ABSTRACT
OBJECTIVES:
T cell immunity plays a critical role in host immune surveillance of tumour cell growth and metastatic spread. This study used small hairpin (sh)RNA-mediated gene silencing to target VTCN1 (B7-H4) expression in a nonsmall-cell lung cancer (NSCLC) cell line (A549) and evaluated the effects on T cell immune activity using an in vitro coculture system.METHODS:
VTCN1-specific shRNA-expressing plasmid was transfected into A549 cells. Mock transfected and empty plasmid-transfected A549 cells served as controls. VTCN1 expression in A549 cells was determined by reverse transcription-polymerase chain reaction (RT-PCR) for VTCN1 mRNA and Western blotting for B7-H4 protein. Transfected A549 cells were cocultured with Jurkat cells. Jurkat cells were examined for proliferation, apoptosis, cell cycle distribution and intracellular cytokine mRNA and protein levels.RESULTS:
VTCN1-specific shRNA efficiently knocked down VTCN1 mRNA and B7-H4 protein levels in A549 cells. This downregulation led to enhanced Jurkat cell proliferation, decreased apoptosis, stimulated cell cycle progression and elevated production of interferon-γ, interleukin (IL)-10 and IL-2.CONCLUSIONS:
B7-H4 negatively regulates T cell-mediated antitumour immunity in NSCLC.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Carcinoma Pulmonar de Células não Pequenas
/
Inibidor 1 da Ativação de Células T com Domínio V-Set
/
Imunidade Celular
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article