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Dextran-catechin conjugate: a potential treatment against the pancreatic ductal adenocarcinoma.
Vittorio, Orazio; Cirillo, Giuseppe; Iemma, Francesca; Di Turi, Giovanni; Jacchetti, Emanuela; Curcio, Michele; Barbuti, Serena; Funel, Niccola; Parisi, Ortensia Ilaria; Puoci, Francesco; Picci, Nevio.
Afiliação
  • Vittorio O; NEST Scuola Normale Superiore & Istituto Nanoscienze-CNR Piazza San Silvestro, 56126 Pisa, Italy.
Pharm Res ; 29(9): 2601-14, 2012 Sep.
Article em En | MEDLINE | ID: mdl-22622510
PURPOSE: A polysaccharide-flavonoid conjugate was developend and proposed for the treatment of pancreatic ductal adenocarcinoma (PDAC). METHODS: The conjugate was synthesized by free radical grafting reaction between catechin and dextran. The chemical characterization of the conjugate was obtained by UV-Vis, 1H-NMR, FT-IR and GPC analyses, while the functionalization degree was determined by the Folin-Ciocalteu assay. The biological activity of the catechin-dextran conjugate was tested on two different cell lines derived from human pancreatic cancer (MIA PaCa-2 and PL45 cells), and the toxicity towards human pancreatic nestin-expressing cells evaluated. RESULTS: Both the cancer cell lines are killed when exposed to the conjugate, and undergo apoptosis after the incubation with catechin-dextran which resulted more effective in killing pancreatic tumor cells compared to the catechin alone. Moreover, our experimental data indicate that the conjugate was less cytotoxic to human pancreatic nestin-expressing cells which are considered a good model of non-neoplastic pancreatic cells. CONCLUSION: The suitability of newly synthesized Dextran-Catechin conjugate in the treatment of PDAC was proved confirming the high potential application of the proposed macromolecula system in the cancer therapy.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Catequina / Dextranos / Carcinoma Ductal Pancreático / Antineoplásicos Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Catequina / Dextranos / Carcinoma Ductal Pancreático / Antineoplásicos Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article