HLA-DRß1*04 typing by simple in-house PCR-SSP technique for rheumatoid arthritis patients.
Rheumatol Int
; 33(4): 867-70, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-22623274
ABSTRACT
A strong association between rheumatoid arthritis (RA) and human leukocyte antigen (HLA) has been observed in many different populations and that accounts for approximately one-third of the genetic component of RA susceptibility. The greatest effect comes from DRß1 gene where the strongest association has been found with DRß1*04 (DR4) allele. As serology has some disadvantages over polymerase chain reaction (PCR)-based techniques and commercially available PCR-based kits are expensive, this study was aimed to standardize simple in-house PCR-SSP technique. Accuracy of this test was further checked with standard PCR-SSOP (RLS) results. The frequency HLA-DRß1*04 was significantly increased among RA patients when compared with normal controls. In this study, a very simple, convenient and more cost-effective in-house PCR-SSP technique was standardized for HLA-DRß1*04 typing that is helpful to RA diagnosis in developing countries like India, which can be used as a good screening test.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artrite Reumatoide
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Teste de Histocompatibilidade
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Cadeias HLA-DRB1
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
/
Middle aged
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article