In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1.
Development
; 139(13): 2447-51, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22627286
ABSTRACT
Neural stem/progenitor cell (NSC/NPC) cultures can be a source of dopamine (DA) neurons for experimental and transplantation purposes. Nurr1, a steroid receptor transcription factor, can overcome the limitations associated with differentiation of cultured NPCs into DA neurons. However, forced Nurr1 expression in NPC cultures generates non-neuronal and/or immature DA cells. We show here that the Nurr1 level and period of expression crucially affect the differentiation and maturation of Nurr1-induced DA neurons. Mature DA neurons were generated by manipulating Nurr1 expression patterns to resemble those in the developing midbrain.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mesencéfalo
/
Neurogênese
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Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares
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Neurônios Dopaminérgicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article