Improving piezoelectric cell printing accuracy and reliability through neutral buoyancy of suspensions.
Biotechnol Bioeng
; 109(11): 2932-40, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22627805
ABSTRACT
The sedimentation and aggregation of cells within inkjet printing systems has been hypothesized to negatively impact printer performance. The purpose of this study was to investigate this influence through the use of neutral buoyancy. Ficoll PM400 was used to create neutrally buoyant MCF-7 breast cancer cell suspensions, which were ejected using a piezoelectric drop-on-demand inkjet printing system. It was found that using a neutrally buoyant suspension greatly increased the reproducibility of consistent cell counts, and eliminated nozzle clogging. Moreover, the use of Ficoll PM400 was shown to not affect cellular viability. This is the first demonstration of such scale and accuracy achieved using a piezoelectric inkjet printing system for cellular dispensing.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Tecidual
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article