Introduction of a non-natural amino acid into a nonribosomal peptide antibiotic by modification of adenylation domain specificity.
Angew Chem Int Ed Engl
; 51(29): 7181-4, 2012 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-22711659
ABSTRACT
Calcium-dependent antibiotics (CDA) are cyclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes. Active site modification of the 3-methyl glutamate activating adenylation (A) domain of the CDA NRPS enables the incorporation of synthetic 3-methyl glutamine into CDA. This provides the first example of how A-domains can be engineered to introduce synthetic "non-natural" amino acids into nonribosomal peptides.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Peptídeos Cíclicos
/
Cálcio
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Streptomyces coelicolor
/
Lipopeptídeos
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Glutamina
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Antibacterianos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article