Multicolor two-photon tissue imaging by wavelength mixing.
Nat Methods
; 9(8): 815-8, 2012 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-22772730
ABSTRACT
We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fótons
/
Cor
/
Microscopia de Fluorescência por Excitação Multifotônica
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article