A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells.
Chin J Integr Med
; 18(7): 502-6, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22772912
ABSTRACT
OBJECTIVE:
To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304.METHODS:
ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.RESULTS:
At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.CONCLUSION:
XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Neovascularização Fisiológica
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Análise de Sequência com Séries de Oligonucleotídeos
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Células Endoteliais
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Indutores da Angiogênese
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article