[Detection of differentially expressed lumican gene mRNA level in scleral tissue in human eye].

ABSTRACT

OBJECTIVE: To identify differentially expressed lumican in scleral tissue of eyes with primary open-angle glaucoma (POAG) and high myopia (HM). METHODS: Total RNA was isolated from scleral tissue of cadaveric eyes derived from normal donors, patient's eyes with diagnosed glaucoma and high myopia who accepted trabeculectomy. RNA was amplified, RT-PCR was used to measure the levels of mRNA. The ratio of the electrophoresis strips'gray scale values of the ß-actin over the lumican gene was obtained for ANOVA analysis. RESULTS: ß-actin/LUM of normal eye was significantly higher than that of POAG and POAG + HM eyes (P < 0.01), but there was no significant difference between POAG and POAG + HM eyes (P > 0.05). CONCLUSIONS: Differentially expressed lumican between POAG and control groups identified in this study have not been previously investigated for their role in the pathogenesis of POAG and thus are novel factors for further study of the mechanism of the disease and for their possible use as diagnostic markers.