[Chemical constituents contained in Populus tomentosa].

OBJECTIVE: To separate and identify chemical constituents from stem barks of male plants of Populus tomentosa. METHOD: Fresh stem barks of P. tomentosa were extracted with methanol to obtain extracts which were suspended in water and blended successively with petroleum ether, ethyl acetate and n-butanol. Various chromatographic techniques were used to separate and purify the constituents extracted with ethyl acetate and n-butanol fractions. Their structures were identified on the basis of their physicochemical properties and spectral data. RESULT: Twelve compounds were separated with ethyl acetate and n-butanol fractions and identified as benzoic acid (1), daucosterol (2), tremuloidin (3), rhamnocitrin (4), sakuranetin (5), 7-O-methylaromadendrin (6), isograndidentatin A (7), siebolside B (8), sakuranin (9), micranthoside (10), alpha-D-glucopyranose (11), and sucrose (12). CONCLUSION: Compounds 4-12 were separated from this plant for the first time. Of them, compound 10 was separated from this plant genus for the first time.