Influenza virus binds its host cell using multiple dynamic interactions.
Proc Natl Acad Sci U S A
; 109(34): 13626-31, 2012 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-22869709
ABSTRACT
Influenza virus belongs to a wide range of enveloped viruses. The major spike protein hemagglutinin binds sialic acid residues of glycoproteins and glycolipids with dissociation constants in the millimolar range [Sauter NK, et al. (1992) Biochemistry 319609-9621], indicating a multivalent binding mode. Here, we characterized the attachment of influenza virus to host cell receptors using three independent approaches. Optical tweezers and atomic force microscopy-based single-molecule force spectroscopy revealed very low interaction forces. Further, the observation of sequential unbinding events strongly suggests a multivalent binding mode between virus and cell membrane. Molecular dynamics simulations reveal a variety of unbinding pathways that indicate a highly dynamic interaction between HA and its receptor, allowing rationalization of influenza virus-cell binding quantitatively at the molecular level.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Orthomyxoviridae
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article