Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos.
Small
; 9(9-10): 1734-41, 2013 May 27.
Article
em En
| MEDLINE
| ID: mdl-22887936
ABSTRACT
The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 µg mL(-1) . However, 30 µg mL(-1) needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 µg mL(-1) needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 µg mL(-1) ) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixes-Gato
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Peixe-Zebra
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Linfócitos B
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Linfócitos T
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Durapatita
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Embrião não Mamífero
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Nanopartículas
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article