Development of a pH-activatable fluorescent probe and its application for visualizing cellular pH change.
Analyst
; 137(19): 4418-20, 2012 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-22888494
ABSTRACT
Novel pH-activatable fluorescent probes with various pK(a) values have been developed utilizing 4-nitro-benzo[1,2,5]oxadiazole derivatives (NBD) as fluorophores and piperazine moieties as proton receptors. Under acidic conditions, probe FoPz displayed significant fluorescent enhancement of about 30 fold with a pK(a) value of 5.70 and it responds rapidly and sensitively to intracellular pH distributions and cellular pH fluctuations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia Confocal
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article