Autonomous screening of C. elegans identifies genes implicated in synaptogenesis.
Nat Methods
; 9(10): 977-80, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22902935
ABSTRACT
Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Caenorhabditis elegans
/
Neurogênese
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article