Nucleotide sequencing and phylogenetic analysis of the 3' region of glycoprotein C gene of South American bovine herpesviruses 1 and 5.
Res Vet Sci
; 94(1): 178-85, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-22929314
ABSTRACT
We herein describe a molecular analysis based on the 3' region of the glycoprotein C gene of 45 bovine herpesvirus (BoHV) isolates from Brazil (n=41), Uruguay (n=2) and Argentina (n=2). Nucleotide (nt) sequencing and alignment of 333 nt revealed levels of similarity ranging from 99.1% to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The isolate SV 453/93 (BoHV-1 associated with genital disease) could not be included within BoHV-1 subtypes since it presented a markedly distinct nt and amino acid (aa) deduced sequences. A transmembrane domain of 24 aa and the putative cytoplasmic tail of 8 aa were identified and mapped. These results indicate that this genome region represents a suitable target for phylogenetic subgrouping of BoHV-1 and BoHV-5 isolates and, perhaps, for understanding evolutionary relationships.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Herpesvirus Bovino 1
/
Herpesvirus Bovino 5
Limite:
Animals
País/Região como assunto:
America do sul
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Argentina
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Brasil
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Uruguay
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article