Temporal association of HLA-B*81:01- and HLA-B*39:10-mediated HIV-1 p24 sequence evolution with disease progression.
J Virol
; 86(22): 12013-24, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22933291
ABSTRACT
HLA-B*8101 and HLA-B*3910 alleles have been associated with viremic control in HIV-1 subtype C infection. Both alleles restrict the TL9 epitope in p24 Gag, and cytotoxic-T-lymphocyte (CTL)-mediated escape mutations in this epitope have been associated with an in vitro fitness cost to the virus. We investigated the timing and impact of mutations in the TL9 epitope on disease progression in five B*8101- and two B*3910-positive subtype C-infected individuals. Whereas both B*3910 participants sampled at 2 months postinfection had viruses with mutations in the TL9 epitope, in three of the five (3/5) B*8101 participants, TL9 escape mutations were only detected 10 months after infection, taking an additional 10 to 15 months to reach fixation. In the two remaining B*8101 individuals, one carried a TL9 escape variant at 2 weeks postinfection, whereas no escape mutations were detected in the virus from the other participant for up to 33 months postinfection, despite CTL targeting of the epitope. In all participants, escape mutations in TL9 were linked to coevolving residues in the region of Gag known to be associated with host tropism. Late escape in TL9, together with coevolution of putative compensatory mutations, coincided with a spontaneous increase in viral loads in two individuals who were otherwise controlling the infection. These results provide in vivo evidence of the detrimental impact of B*8101-mediated viral evolution, in a single Gag p24 epitope, on the control of viremia.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos HLA-B
/
Infecções por HIV
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HIV-1
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Proteína do Núcleo p24 do HIV
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article