Transcriptomics using next generation sequencing technologies.
Methods Mol Biol
; 917: 293-317, 2012.
Article
em En
| MEDLINE
| ID: mdl-22956096
ABSTRACT
Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xenopus
/
Análise de Sequência de DNA
/
Perfilação da Expressão Gênica
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Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article