A reaction-based fluorescent probe for selective imaging of carbon monoxide in living cells using a palladium-mediated carbonylation.
J Am Chem Soc
; 134(38): 15668-71, 2012 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-22970765
ABSTRACT
Carbon monoxide is a member of the gasotransmitter family, which also includes NO and H(2)S, and has been implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. Here we report a new type of turn-on fluorescent probe for selective CO detection based on palladium-mediated carbonylation reactivity. CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paládio
/
Compostos de Boro
/
Monóxido de Carbono
/
Complexos de Coordenação
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article