Constitutive autotaxin transcription by Nmyc-amplified and non-amplified neuroblastoma cells is regulated by a novel AP-1 and SP-mediated mechanism and abrogated by curcumin.
FEBS Lett
; 586(20): 3681-91, 2012 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-22975311
ABSTRACT
The motility, angiogenesis and metastasis-stimulating factor Autotaxin (Atx), over expressed by human neuroblastomas (NB), is constitutively expressed by human Nmyc-amplified SK-N-BE and non-Nmyc-amplified SH-SY5Y NB cells. Here, we characterise a novel Atx transcriptional mechanism, utilised by both cell lines, that is restricted to the first 285bp of the Atx promoter and involves AP-1 and SP transcription factors, acting through a CRE/AP-1-like element at position -142 to -149 and a GAbox at position -227 to -235 relative to the Atx translational start site. This novel transcriptional mechanism can be inhibited by internally initiated SP-3 and the natural phenol curcumin.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Proteínas Proto-Oncogênicas c-myc
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Diester Fosfórico Hidrolases
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Fator de Transcrição AP-1
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Curcumina
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Fatores de Transcrição Sp
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Neuroblastoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article