[Expression of inducible nitric oxide synthase in restenosis rats and function of Astragalus membranaceus and Angelica sinensis].

ABSTRACT

OBJECTIVE: To explore the expression of inducible nitric oxide synthase (iNOS) in restenosis rats and function of Astragalus membranaceus and Angelica sinensis. METHOD: The restenosis model was established by denuding aorta endothelium, rats were randomly divided into control group, model group, A. membranaceus treatment group, A. sinensis treatment group, combined A. membranaceus with A. sinensis treatment group. After intramuscular injection of drugs for 21 dayss, the changes of iNOS in restenosis rats were observed by histomorphology and immunohistochemisty, the effects of A. membranaceus and A. sinensis on iNOS in restenosis rats was also investigated. RESULT: A small quantity of iNOS were detected in the intima and media of normal aorta, the expression of iNOS was increased on 3 day after denuding aorta endothelium, the expression of iNOS increasd and the color darken along with injury damage and intima thickening. Compared with model group, the expression of iNOS decreasd in A. membranaceus, A. sinensis treated group, A. membranaceus and A. sinensis treated group changed more significantly. CONCLUSION: iNOS was involved in blood vessel restenosis by denuding aorta endothelium, A. membranaceus, A. sinensis could inhibit intimal proliferation through iNOS.