A generalized mathematical model to estimate T- and B-cell receptor diversities using AmpliCot.
Biophys J
; 103(5): 999-1010, 2012 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-23009849
ABSTRACT
The efficiency of the adaptive immune system is dependent on the diversity of T- and B-cell receptors, which is created by random rearrangement of receptor gene segments. AmpliCot is an experimental technique that allows the measurement of the diversity of the T- and B-cell repertoire. This procedure has the advantage over other cloning and sequencing techniques of being time- and expense-effective. In previous studies, receptor diversity, measured with AmpliCot, has been inferred assuming a second-order kinetics model. The latter implies that the relation between diversity and concentration × time (Cot) values is linear. We show that a more detailed model, involving heteroduplex and transient-duplex formation, leads to significantly better fits of experimental data and to nonlinear diversity-Cot relations. We propose an alternative fitting procedure, which is straightforward to apply and which gives an improved description of the relationship between Cot values and diversity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos B
/
Receptores de Antígenos de Linfócitos T
/
Modelos Biológicos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article