Extending the biotic ligand model to account for positive and negative feedback interactions between cadmium and zinc in a freshwater alga.
Environ Sci Technol
; 46(21): 12129-36, 2012 Nov 06.
Article
em En
| MEDLINE
| ID: mdl-23030358
ABSTRACT
Low concentrations of essential trace metals such as zinc (Zn) were recently shown to strongly modulate cadmium (Cd) uptake in the freshwater alga Chlamydomonas reinhardtii. Here we studied the mechanisms of Cd and Zn acquisition by this alga, using metal uptake kinetics experiments. Cadmium uptake rates fitted a three transport site model characterized by the affinity constants K(Cd1)(Cd) = 10(5.0), K(Cd2)(Cd) = 10(7.6), and K(Cd3)(Cd) = 10(8.8). Similar uptake kinetics were obtained for Zn with K(Zn1)(Zn) = 10(5.0), K(Zn2)(Zn) = 10(7.4), and K(Zn3)(Zn) > 10(9). Competitive binding experiments suggest that Zn and Cd share the same three transport systems. The capacities of the transport systems were modulated by as much as 10-fold following preacclimation to high or low Zn(2+) and Cd(2+) concentrations. We conclude that the strong protective effect of Zn on Cd accumulation is mainly due to the reduction of the maximal uptake rate of the high-affinity Zn2 (or Cd2) transport system. A biotic ligand model was developed to incorporate the effects of both chemical speciation and physiological regulation of Cd transport systems. The model successfully predicts the experimentally measured steady-state Cd content of C. reinhardtii in the presence of low or high [Zn(2+)].
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
/
Zinco
/
Cádmio
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Chlamydomonas reinhardtii
/
Modelos Biológicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article