Purification of RNA expressed in vivo inserted in a tRNA scaffold.
Methods Mol Biol
; 941: 1-8, 2012.
Article
em En
| MEDLINE
| ID: mdl-23065549
ABSTRACT
For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. In vitro transcription or chemical synthesis are the principal methods to produce RNA. Here, we describe an alternative method allowing RNA production in bacteria and its purification by liquid chromatography. In a few days, between 10 and 100 mg of pure RNA are obtained with this technique.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Engenharia Genética
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article